Qiaamp Dna Blood Mini Kit 250 Protocol
No claim or representation is intended for their use to identify any specific organism or for clinical use diagnostic prognostic therapeutic or blood banking.
Qiaamp dna blood mini kit 250 protocol. No claim or representation is intended to provide information for the diagnosis prevention or treatment of a disease. The qiaamp dna mini kit provides silica membrane based nucleic acid purification from tissues swabs csf blood body fluids or washed cells from urine. Warnings precautions a. Purification of high molecular weight dna 24 dna purification protocols purification of total dna from animal blood or cells.
Of hbv positive serum using the qiaamp dna blood mini kit qiaamp or an sds phenol method sds phenol and analyzed by agarose gel electrophoresis. The flexigene dna kit provides scalable purification of genomic dna from whole blood buffy coat or cultured cells in a single tube. Use standard precautions when handling all body fluids tissues and cell cultures. Qiaamp dna blood midi and maxi kits are available for purification of dna from up to 2 ml and 10 ml of blood respectively.
Qiaamp dna mini and blood mini handbook 05 2016 5 kit contents qiaamp dna kits blood mini 50 blood mini 250 mini 50 mini 250 catalog no. No mechanical homogenization is necessary as the tissues are lysed enzymatically. M1 00 10 1 10 2 qiaamp 10 3 m1 00 10 1 10 2 sds phenol 10 3 qiaamp procedures lyse blood tissues body uids bind wash elute pure genomic or viral dna automatable on. 69504 69506 number of preps 50 250 dneasy mini spin columns.
Refer to the specimen handling and collection procedure gen 1 for guidelines specific for the molecular genetics laboratory and samples. Qiaamp dna mini and blood mini handbook 11 2007 7 product use limitations the qiaamp dna mini kit and qiaamp dna blood mini kit are intended for general laboratory use. The typical yield from 200 µl healthy whole blood is 4 12 µg with an elution volume of 50 200 µl. It is the user s responsibility to validate the.
6 qiaamp dna mini kit and qiaamp dna blood mini kit 09 2001 storage conditions qiaamp spin columns and buffers can be stored dry at room temperature 15 25 c for up to 1 year without showing any reduction in performance. Dneasy blood tissue kit 50 250 catalog no. The convenient spin column procedure reduces hands on preparation time to 20 minutes. 51104 51106 51304 51306 number of preps 50 250 50 250 qiaamp mini spin columns 50 250 50 250 collection tubes 2 ml 150 750 150 750 buffer al 12 ml 2 x 33 ml 12 ml 2 x 33 ml.
The qiaamp dna blood mini kit processes sample sizes of up to 200 µl with a preparation time of 20 40 minutes. These kits use the same silica membrane technology as the qiaamp dna blood mini kit. The qiaamp dna blood mini kit procedure is automatable see figure qiacube. Qiaamp dna mini kit and qiaamp dna blood mini kit 02 2003 7 product use limitations qiaamp kits are intended as general purpose devices.