Qiaamp Dna Blood Mini Kit Protocol

This protocol is used to extract gdna from a frozen cell pellet of less than 5e6 cells using the qiaamp dna blood mini kit qiagen cat 51104.

Qiaamp dna blood mini kit protocol. Zero bias scores article reviews protocol conditions and more. M1 00 10 1 10 2 qiaamp 10 3 m1 00 10 1 10 2 sds phenol 10 3 qiaamp procedures lyse blood tissues body uids bind wash elute pure genomic or viral dna automatable on. Qiaamp dna mini and blood mini handbook 11 2007 7 product use limitations the qiaamp dna mini kit and qiaamp dna blood mini kit are intended for general laboratory use. Qiaamp dna mini kit and qiaamp dna blood mini kit 02 2003 7 product use limitations qiaamp kits are intended as general purpose devices.

These kits use the same silica membrane technology as the qiaamp dna blood mini kit. Isolation of genomic dna mini kit purpose. Qiaamp dna mini blood mini handbook en. Qiaamp dna blood midi and maxi kits are available for purification of dna from up to 2 ml and 10 ml of blood respectively.

No claim or representation is intended to provide information for the diagnosis prevention or treatment of a disease. Print bookmark share. Lyophilized qiagen protease can be stored at room temperature 15 25 c for up to 6 months without any decrease in performance. 99 100 based on 1874 pubmed citations.

The qiaamp dna blood mini kit is designed for processing up to 200 μl fresh or frozen human whole blood. The qiaamp dna blood mini kit provides silica membrane based dna purification. The flexigene dna kit provides scalable purification of genomic dna from whole blood buffy coat or cultured cells in a single tube. Set the water bath to 70oc and thaw cell pellets on ice.

Qiaamp mini spin columns can be easily processed in a centrifuge or on vacuum manifolds. Qiagen conventional qiaamp dna mini blood kit based extraction procedure conventional qiaamp dna mini blood kit based extraction procedure supplied by qiagen used in various techniques. Of hbv positive serum using the qiaamp dna blood mini kit qiaamp or an sds phenol method sds phenol and analyzed by agarose gel electrophoresis. It is the user s responsibility to validate the.