Qiaamp Dna Stool Mini Kit Protocol Pdf
6 quality control 7 introduction 8 purification of viral rna and dna 8 purification of dna from urine 9 purification of dna from stool 9 purification of dna from formalin fixed.
Qiaamp dna stool mini kit protocol pdf. En qiaamp dna stool handbook. Alcohol is added and lysates loaded onto the qiaamp spin column. No claim or representation is intended for their use to identify any specific organism or for clinical use diagnostic prognostic therapeutic or blood banking. See appendix f page 58.
Special protocols for. The qiaamp dna stool mini kit simplifies isolation of dna from stool with a fast spin column procedure see flowchart procedure. Efficient lysis with no mechanical homogenization only 20 minutes of total hands on preparation time purification of high quality dna from a wide range of samples the qiaamp dna mini kit can be used to isolate dna from human tissues swabs blood or washed cells from urine. P1 protocol revised 17 jan 2020 bbms3004 dna extraction protocol 2020 pdf.
Print bookmark share for dna purification from stool samples pdf 347kb english format file size language download get. Faecal genomic dna was extracted by two phenol chloroform extraction methods and a commercial kit qiaamp dna stool mini kit and gdna extracted from rumen using glass milk extraction method. Qiaamp dna mini kit and qiaamp dna blood mini kit 02 2003 7 product use limitations qiaamp kits are intended as general purpose devices. Optimized buffers and enzymes lyse samples stabilize nucleic acids and enhance selective dna adsorption to the qiaamp membrane.
The qiaamp dna mini kit provides. No claim or representation is intended to provide information for the diagnosis prevention or treatment of a disease. Lyophilized qiagen protease can be stored at room temperature 15 25 c for up to 6 months without any decrease in performance. Qiaamp dna mini or qiaamp dna blood mini kits can also be used to purify viral dna from stool but removal of inhibitors is not as effective.
Qiaamp dna mini and blood mini handbook 11 2007 7 product use limitations the qiaamp dna mini kit and qiaamp dna blood mini kit are intended for general laboratory use. It is the user s responsibility to validate the. View qiaamp dna mini kit gdna extraction kit.