Qiaamp Dna Mini Kit 50 Protocol
Dna purified using the qiaamp dna mini kit can be used in a wide range of downstream applications including pcr and quantitative real time pcr southern blotting snp and str genotyping and pharmocogenomic research.
Qiaamp dna mini kit 50 protocol. Click on the product or protocol links to be directed to the manufacturers pages. Qiaamp dna mini kit. Qiaamp dna mini kit 51304 51306 1113198. 51104 51106 51304 51306 number of preps 50 250 50 250 qiaamp mini spin columns 50 250 50 250 collectiontubes 2 ml 150 750 150 750 buffer al 12 ml 2 x 33 ml 12 ml 2 x 33 ml buffer atl 14 ml 50 ml buffer aw1 concentrate 19 ml 98 ml 19 ml 98 ml.
It is the user s responsibility to validate the. Efficient lysis with no mechanical homogenization only 20 minutes of total hands on preparation time purification of high quality dna from a wide range of samples the qiaamp dna mini kit can be used to isolate dna from human tissues swabs blood or washed cells from urine. Lyophilized qiagen protease can be stored at room temperature 15 25 c for up to 6 months without any decrease in performance. 6 qiaamp dna mini kit and qiaamp dna blood mini kit 09 2001 storage conditions qiaamp spin columns and buffers can be stored dry at room temperature 15 25 c for up to 1 year without showing any reduction in performance.
All due care and attention should be exercised in the handling of the products. No claim or representation is intended for their use to identify any specific organism or for clinical use diagnostic prognostic therapeutic or blood banking. Set the water bath to 70oc and thaw cell pellets on ice. This protocol is used to extract gdna from a frozen cell pellet of less than 5e6 cells using the qiaamp dna blood mini kit qiagen cat 51104.
These products are not intended for the diagnosis prevention or treatment of a disease. No claim or representation is intended to provide information for the diagnosis prevention or treatment of a disease. Qiaamp dna mini kit vs quick dna universal kit 50 preps this page will help you compare kit specs and protocols across manufacturers. The qiaamp dna mini kit yields dna sized up to 50 kb see figure purification of up to 50 kb genomic dna.
Qiaamp dna kits blood mini 50 blood mini 250 mini 50 mini 250 catalog no. We recommend all users of qiagen products to adhere to the nih guidelines. Special protocols for. Qiaamp dna mini and blood mini handbook 11 2007 7 product use limitations the qiaamp dna mini kit and qiaamp dna blood mini kit are intended for general laboratory use.
The qiaamp dna mini kit and qiaamp dna blood mini kit are intended for molecular biology applications.